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• Cover
• Title Page
• Copyright
• About the Companion Website
• Preface to the Sixth Edition
• 1 The Development of Molecular Biotechnology
• Emergence of Molecular Biotechnology
• Recombinant DNA Technology
• Commercialization of Molecular Biotechnology
• Concerns and Consequences
• Summary
• References
• Review Questions
• 2 Fundamental Technologies
• Molecular Cloning
• Genome Engineering Using CRISPR Technology
• Polymerase Chain Reaction
• Chemical Synthesis of Genes
• DNA Sequencing Technologies
• Sequencing Whole Genomes
• Genomics
• Summary
• References
• Review Questions
• 3 Production of Recombinant Proteins
• Protein Production in Prokaryotic Hosts
• Heterologous Protein Production in Eukaryotic Cells
• Protein Engineering
• Summary
• References
• Review Questions
• 4 Molecular Diagnostics
• Immunological Approaches To Detect Protein Biomarkers
• DNA‐Based Diagnostic Approaches
• Detecting RNA Signatures of Disease
• Biofluorescent and Bioluminescent Systems
• Summary
• References
• Review Questions
• 5 Protein Therapeutics
• Pharmaceuticals
• Enzymes
• Bacteria and Therapeutics
• Recombinant Antibodies
• Affibody Molecules
• Summary
• References
• Review Questions
• 6 Nucleic Acids as Therapeutic Agents
• Targeting Specific mRNA and DNA Sequences
• Viral Delivery Systems
• Nonviral Delivery Systems
• Gene Therapy
• Summary
• References
• Review Questions
• 7 Vaccines
• Vaccination
• Current and Future Vaccines
• Subunit and Peptide Vaccines
• Genetic Immunization: DNA Vaccines
• Engineered Attenuated Vaccines
• Vector Vaccines
• Monoclonal Antibody Passive Immunity
• Summary
• References
• Review Questions
• 8 Industrial and Environmental Uses of Recombinant Microorganisms
• Restriction Endonucleases
• Small Biological Molecules
• Microbial Degradation of Xenobiotics
• Utilization of Starch and Sugars
• Utilization of Cellulose and Hemicellulose
• Lipids from Cyanobacteria
• Hydrogen Production
• Summary
• References
• Review Questions
• 9 Large‐Scale Production of Proteins and Nucleic Acids from Recombinant Microorganisms
• Principles of Microbial Growth
• Maximizing the Efficiency of the Fermentation Process
• Bioreactors
• Typical Large‐Scale Fermentation Systems
• Harvesting Microbial Cells
• Disrupting Microbial Cells
• Downstream Processing
• Large‐Scale Production of DNA and RNA
• Summary
• References
• Review Questions
• 10 Genetic Engineering of Plants: Methodology
• Plant Transformation with the Ti Plasmid of A. tumefaciens
• Ti Plasmid‐Derived Vector Systems
• Microprojectile Bombardment
• Chloroplast Engineering
• Use of Reporter Genes in Transformed Plant Cells
• Manipulation of Gene Expression in Plants
• Production of Marker‐Free Transgenic Plants
• Summary
• References
• Review Questions
• 11 Transgenic Plants
• Insect Resistance
• Virus Resistance
• Herbicide Resistance
• Fungus and Bacterium Resistance
• Salt and Drought Stress
• Phytoremediation
• Fruits and Flowers
• Modification of Plant Nutritional Content
• Modification of Food Plant Taste and Appearance
• Plants as Bioreactors
• Edible Vaccines
• Plant Yield
• Summary
• References
• Review Questions
• 12 Transgenic Animals
• Transgenic Animal Methodologies
• Transgenic Animal Models of Human Diseases
• Animal Bioreactors for Production of Recombinant Therapeutic Proteins
• Enhancing Production Traits of Food Animals
• Gene Drives To Eradicate Vector‐Transmitted Diseases
• Summary
• References
• Review Questions
• 13 Molecular Biotechnology and Society
• Development of Guidelines for Recombinant DNA Research
• Deliberate Release of Genetically Modified Microorganisms
• Regulation of Genetically Modified Foods
• Societal Concerns about Genetically Modified Foods
• Regulation and Safety of Medical Products of Biotechnology
• Patenting Biotechnology
• Summary
• References
• Review Questions
• Amino Acids of Proteins and Their Designations
• Index
• End User License Agreement