Efnisyfirlit

• Cover
• Preface
• About the Authors
• SECTION I: The Biology behind the Technology
• 1 Fundamental Technologies
• Molecular Cloning
• Genomic Libraries
• Amplification of DNA Using PCR
• DNA Sequencing Technologies
• Sequencing Whole Genomes
• Genomics
• summary
• review questions
• references
• 2 Fundamental Concepts in Immunology
• The Immune Response
• Functions of the Immune System
• Cells of the Immune System: Cell-Mediated Immunity
• Tissues of the Immune System
• Humoral Immunity
• Types of Antibodies: Applications
• Immunological Techniques
• summary
• review questions
• references
• 3 The Genetic Basis of Disease
• Chromosomal Disorders and Gene Mapping
• Single-Gene Disorders
• Polygenic Disorders and Gene Clustering
• Mitochondrial Disorders
• summary
• review questions
• references
• 4 Immune Pathogenesis
• Models of Immune System Lesions
• Inflammation and Immune Hypersensitivity Disorders
• Immunodeficiency Disorders and Defects in Development of the Immune System
• summary
• review questions
• references
• 5 Microbial Pathogenesis
• Introduction
• Bacterial Infections
• Viral Infections
• summary
• review questions
• references
• SECTION II: Production of Therapeutic Agents
• 6 Modulation of Gene Expression
• Manipulating Gene Expression in Prokaryotes
• Heterologous Protein Production in Eukaryotic Cells
• Directed Mutagenesis
• summary
• review questions
• references
• 7 Genetic Engineering of Plants
• Plant Transformation with the Ti Plasmid of A. tumefaciens
• Physical Transfer of Genes to Plants
• Chloroplast Engineering
• Transient Gene Expression
• Molecular Pharming
• Edible Vaccines
• summary
• review questions
• references
• SECTION III: Diagnosing and Treating Human Disease
• 8 Molecular Diagnostics
• Immunological Approaches To Detect Protein Biomarkers of Disease
• DNA-Based Approaches to Disease Diagnosis
• Detecting RNA Signatures of Disease
• summary
• review questions
• references
• 9 Protein Therapeutics
• Pharmaceuticals
• Recombinant Antibodies
• Enzymes
• Lactic Acid Bacteria
• summary
• review questions
• references
• 10 Nucleic Acid Therapeutic Agents and Human Gene Therapy
• Treating Genetic and Nongenetic Disorders
• Targeting Specific mRNAs and DNAs
• Viral Delivery Systems
• Nonviral Nucleic Acid Delivery Systems
• Gene Therapy
• summary
• review questions
• references
• 11 Vaccines
• Vaccination: Overview
• Subunit Vaccines
• Peptide Vaccines
• Dendritic Cell Vaccines
• DNA Vaccines
• Attenuated Vaccines
• Vector Vaccines
• Adjuvants
• Systems Biology and Evaluation of Vaccines
• summary
• review questions
• references
• 12 Societal Issues
• Safety and Ethical Issues
• Patenting Biotechnology
• Economic Issues
• summary
• review questions
• references
• Glossary
• Index
• End User License Agreement